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Anti-Human IgG scFv-HRP

R4,006.50R560,910.00

This antibody recognises and binds specifically to Human Immunoglobulin 1. It is ideal for ELISA and western blot applications and comes as either a full antibody with Human or Rabbit Fc regions or as an scFv conjugated to HRP.

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Description

This antibody recognises and binds specifically to Human Immunoglobulin 1. It is ideal for ELISA and western blot applications and comes as either a full antibody with Human or Rabbit Fc regions or as an scFv conjugated to HRP. The ScFv conjugated to HRP is a favourite as it allows two steps in one, being both a primary and secondary antibody in one!

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OVERVIEW

Product name Anti-Human IgG scFv-HRP Antibody
Host species Mouse variable regions (produced in Nicotiana benthamiana plants)
Target species Human
Production This product is a recombinant single chain variable fragment (scFv) fused to Horseradish Peroxidase (HRP) and produced in N. benthamiana plants
Test applications Tested for: WB, ELISA and dot blot
Immunogen Human IgG, whole molecule
Conjugate Horseradish peroxidase (HRP)
Concentration 1mg/ml
Volume Variable

PROPERTIES

Form Liquid
Colour Clear with Reddish/brown tinge
Preparation instructions None. Store immediately.
Storage Short term: 2-4°C. Long term: -20°C
Storage buffer Constituents: Phosphate Buffered saline (0.1M) pH 7.4
Purification notes This product was purified using affinity chromatography (Ni-IMAC)
Purity 90-95% in relation to other proteins in sample
Conjugation notes Horseradish Peroxidase C (EC 1.11.1.7) recombinantly fused to N-terminus
Clonality Monoclonal
Isotype Single chain variable fragment (scFv)
Stability Up to 1 year from delivery
General notes This recombinantly produced secondary anti-human IgG scFv fused to HRP is optimised for western blot and ELISA use. The reddish/brown colour is common with HRP conjugated antibodies. If for any reason the product fails to give results, please contact our scientific support team for assistance.

APPLICATIONS

This product is guaranteed in the following applications. The application notes below include recommended starting dilutions and conditions, however, optimal dilutions/concentrations should be determined by the end user.
Western blot Suggested working dilution 1/1000-1/5000 to detect 1μg antigen in 5% milk. Suggested incubation conditions:1 hr at 37 C/ 4 C overnight.
ELISA None. Store immediately.
Dot blot Clear with Reddish/brown tinge
Important to Note: This antibody is conformationally specific to native human IgG1.

Performance Data: Coomassie Stained Gel:

Figure 1. Coomassie-stained SDS-PAGE of purified anti-Human IgG scFv-HRP antibody Figure 2. Western blot of HIV-1 p24 recombinant protein expression, detected using the anti-HIV-1 p24 Full (Human) (catalogue number CBP_0002.3) anti-Human IgG scFv-HRP secondary antibody (Cat no CBP_0003.1) Figure 3. ELISA results showing anti-Human IgG scFv-HRP binds to both Human IgG as well as a human anti-TNF alpha antibody. No cross-reactivity observed to a rabbit Fc fusion protein. N/A refers to background buffer control.
Download our MATERIALS SAFETY DATA Sheet for more product information.

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